Knowledge-enriched characterization of cell populations. Morphological information from visuals can add towards the richness of flow cytometry information. As an example, the figure reveals an in any other case typical apoptosis assay making use of Annexin V and PI, introducing mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinct features. These insights could not have already been gained from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and retailers photographs of detected functions in a rate around 6,000 pictures per second, with regards to the flow price and image dimension. For greater flexibility, Attune Cytometric Software program enables you to alter the image seize frequency as desired and select a particular gate for graphic capture, if needed.
(B) Uncooked 264.7 macrophages will often be annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The volume of singlets obtainable for Assessment improves when the user trains and employs a more exact product.
Find out new insights into your experimental knowledge by visualizing populations using mixed fluorescence and morphology information. Our computer software interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment which might be built to the program for an intuitive, streamlined information Examination working experience.
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Moreover, it is actually illuminating to view how many doublets slip through classic singlet gates, and fantastic to confirm the position of unexpected double-good situations in my Assessment."
Optimizing gating strategy. Even sturdy handbook singlet gating is mistake-vulnerable and remains a subjective choice place in Practically all flow cytometry assays. Imaging can be employed to confirm and change gates to incorporate only solitary cells of interest.
Our experts will design and style panels for you as again and again you would like—cost-free, no products order wanted.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also means that you can use morphological features to find exciting subpopulations that will not be obvious read more from flow cytometry information by itself.
Potentially most importantly, these activities incorporate cells of Plainly distinct phenotypes which could lead on to incorrect conclusions pertaining to double constructive events (specifically in unusual populations).
Sound waves empower precise cell alignment, causing Fantastic knowledge excellent and ~10X a lot quicker sample flow premiums.
We Beforehand shown the strength of imaging CAR-T/Ramos cell interactions. Enable’s look at just the inhabitants of biggest interest, the double positive situations, To find out more. We can now use extended morphological parameters (below, circularity and skewness of depth) to additional study the features of these populations and refine gating on these functions, expanding info robustness.
Designed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated population resolution in lysed total human blood. Consumer can perspective events from Every single cluster for Visible affirmation of mobile phenotypes.
Flexible payment plan from Agilent Economic Options help you obtain the important instrumentation you may need for rapid venture, or to develop your lab.
At the guts of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which utilizes seem waves to meticulously align cells inside the flow cell. This innovative approach surpasses traditional hydrodynamic procedures, resulting in more quickly processing speeds, Excellent facts good quality, and amplified sample throughput.
Since the mobile line experienced Formerly been analyzed around the Attune CytPix Flow Cytometer, the scientists went again to the pictures and have been ready to document the microbial an infection at the very least five times previously.
The Attune CytPix Flow Cytometer lets consumers to visualise samples all through acquisition, verify the accuracy of gating techniques, and help benefits with trustworthy documentation for publication purposes.
What seemed to be a homogeneous populace is really far more numerous—an Perception that ought to be regarded as when interpreting success.